24-epibrasinolide modulates the vase life of lisianthus cut flowers by modulating acc oxidase enzyme activity and physiological responses

Date
2021-05
Authors
Darvish, Mohammad
Shirzad, Habib
Asghari, Mohammadreza
Noruzi, Parviz
Alirezalu, Abolfazl
Pateiro, Mirian
Takshe, Aseel A.
Lorenzo, José Manuel
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI AG
Abstract
Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L) and kept at 25◦C for 15 days. The ACC oxidase activity, water absorption, malondialdehyde (MDA) production and vase solution absorption rates, chlorophyll and anthocyanin contents, and the vase life of the flowers were evaluated during and at the end of storage. EBL at 3 µmol/L significantly (p ≤ 0.01) enhanced the flower vase life by decreasing the ACC oxidase activity, MDA production and senescence rates, and enhancing chlorophyll and anthocyanin biosynthesis and accumulation, relative water content, and vase solution absorption rates. By increasing the concentration, EBL negatively affected the flower vase life and postharvest quality probably via enhancing the ACC oxidase enzyme activity and subsequent ethylene production. EBL at 6 and 9 µmol/L and in a concentration dependent manner, enhanced the ACC oxidase activity and MDA production rate and decreased chlorophyll and anthocyanin accumulation and water absorption rate. The results indicate that the effects of brassinosteroids on ethylene production and physiology of lisianthus cut flowers is highly dose dependent. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Description
This article is not available at CUD collection. The version of scholarly record of this article is published in Plants (2021), available online at: https://doi.org/10.3390/plants10050995
Keywords
ACC oxidase, Anthocyanins, Ethylene, Lisianthus, Malondialdehyde, Vase life
Citation
Darvish, M., Shirzad, H., Asghari, M., Noruzi, P., Alirezalu, A., Pateiro, M., Takshe, A., & Lorenzo, J.M. (2021). 24-epibrasinolide modulates the vase life of lisianthus cut flowers by modulating acc oxidase enzyme activity and physiological responses. Plants, 10(5). https://doi.org/10.3390/plants10050995